Figure 3

Lifeact-mTurquoise2 localizes to the nuclear boundary and exhibits a ‘flash’ of Lifeact during nuclear disassembly. A) Time series of a living cell undergoing cell division. Just prior to nuclear disassembly, accumulation of Lifeact is apparent (A2.0-6.0) and during prometaphase disappears with a ‘flash’ (A6.5-7.5). See main text for a more detailed description of the full cleavage cycle. Scale bar 10 μm. B) When the cell rounds up during metaphase-anaphase Lifeact-mTurquoise2 fluorescence appears to increase at the cell boundary through time (different time points are indicated by colored lines). The average profiles perpendicular to the plasma membrane region going from the intracellular space (left) to the extracellular space (right) from the region depicted in A8.0 (yellow line) are normalized to the peak value of the profile at A8.0, show for this cell an increase of about 2.5 fold. C) Quantification of the Lifeact fluorescence at the nuclear boundary from 0.0-6.0 min. The profiles show the averaged normalized fluorescence perpendicular to the nuclear boundary going from nucleoplasm (left) to the cytoplasm (right)