Figure 4

Labeling of F-actin, DNA and tubulin in fixed embryos. A-B) Preserved cells labeled with the F-actin stain, phallicidin-FL (green) exhibit F-actin at the nuclear boundary of cells that are at an early phase of cell division. C) Alpha-tubulin staining (red) shows the formation of centrosomes adjacent to the nucleus. D) DNA staining with Hoechst (blue) show that the nuclear structures are still intact. E) Overlay of all three channels from B-D shows the relative position of all markers. F) Several cells at metaphase stained with the same labels show the fully formed mitotic spindles and the chromosomes that are lined up along the metaphase plate. F-actin associated with the nuclear boundary is no longer present, but is faintly visible in the cytoplasm and clearly at the plasma membrane