Figure 2

The transcripts of target gene Bcl-2 and IgG post-siRNA transfection were detected and immunofuorescent co-localization analysis of Alex488 and Alex555 was performed. (A) The transcripts of target gene Bcl-2 and IgG post-siRNA transfection 48 hr were detected in TEV-1 cells by real time RT-PCR. (B) Immunofluorescence signals for Alex555 (red) were observed with a Zeiss laser confocal microscope at 546 nm emition wavelength in HPG and HPG plus anisomycin treated group. Nuclear staining was performed with DAPI. (C) Immunofuorescent co-localization analysis of Alex488 (green) and Alex555 (red). White dots, as arrows were shown in big magnification, represented newly synthesized Bcl-2 and IgG proteins, respectively. (D) The percentage of immunofluorescence co-localization of Alex488 and Alex555 in TEV-1 cells was detected. Bar: 5 μm; Magnification: 10 ×100. Data are presented as means ± S.E. of three independent experiments (*P < 0.05; **p < 0.01).