Skip to main content
Figure 3 | BMC Cell Biology

Figure 3

From: A morphologic and semi-quantitative technique to analyze synthesis and release of specific proteins in cells

Figure 3

Newly synthesized protein secretion was detected. (A) The schematic diagram illustrated the detection of newly synthesized protein release in the culture supernatant. Newly synthesized proteins from HPG group were labeled with azide-biotin in situ, by clicking its reaction, the biotin signals were incubated with streptavidine and measured by an ELISA reader. (B) The dynamic curve for newly synthesized MMP-9 proteins was set up corresponding to various time points by adapted ELISA method. Normal culture condition was treated as negative control and selected as baseline and named as pulse 0; chase 0. Pulse 1 h, chase 0 h named as pulse 1; chase 0. Pulse 4 h, chase 0 h named as pulse 4; chase 0. Pulse 4 h, chase 0.5 h named as pulse 4; chase 0.5. Pulse 4 h, chase 2 h named as pulse 4; chase 2. Pulse 4 h, chase 4 h named as pulse 4; chase 4. Pulse 4 h, chase 24 h named as pulse 4; chase 24. (C) The dynamics curve for newly synthesized IgG protein was set up corresponding to various time points. Normal culture condition was treated as negative control and selected as baseline, and the named method for various time points was similar to MMP-9.

Back to article page