A morphologic and semi-quantitative technique to analyze synthesis and release of specific proteins in cells

Table 6 siRNA oligos and the primers for real time RT-PCR

Gene	siRNA(5′-3′)	Primer pairs(5′-3′)	Size(bp)
Bcl-2	GUACAUCCAUUAUAAGCUGdtdt	GTACCTGAAACCGGCACCTGCAC	150 bp
Bcl-2	CAGCUUAUAAUGGAUGUACdtdt	GCTCCACAGCCTCCCATTGCC	150 bp
MMP-9	CAUCACCUAUUGGAUCCAAdtdt	CAGCCGGGACGCAGACATCG	128 bp
MMP-9	UUGGAUCCAAUAGGUGAUGdtdt	GGGCGTCTCCCTGAATGCCG	128 bp
IgG	CCAAGGACACCCUCAUGAUdtdt	GTGACGGTGTCGTGGAACT	94 bp
IgG	AUCAUGAGGGUGUCCUUGGdtdt	ACGCTGCTGAGGGAGTAGAG	94 bp
Scramble	UUCUCCGAACGUGUCACGUdtdt
Scramble	ACGUGACACGUUCGGAGAAdtdt
GAPDH		TAGAGCGGCCGCCATGTTGC	123 bp
GAPDH		CCTGACTTCCCCGCCACACGC	123 bp

ISSN: 2661-8850