Skip to main content

Table 1 Relative gene expression (expressed in mean ± standard deviation) for human adipose tissue- (AT-), bone marrow- (BM-) and Wharton’s Jelly-mesenchymal stromal cells (WJ-MSC) when grown in low glucose media supplemented with 10% fetal bovine serum

From: Gene expression and protein secretion during human mesenchymal cell differentiation into adipogenic cells

  BM-MSC AT-MSC WJ-MSC
d10 d21 d10 d21 d10 d21
PPARG 14.9 ± 2.5 a 32.3 ± 3.0 a 21.0 ± 6.4 a 58.3 ± 13.6 b 15.2 ± 8.9 a 29.1 ± 3.2 a
ADIPOQ 4,126.8 ± 3,370.2 a 3,994.2 ± 1,292.7 a 3,378.4 ± 2,243.0 a 423,626.8 ± 147,352.8 b * *
CEBPA 102.6 ± 14.6 a 89.7 ± 33.1 a 206.6 ± 53.9 a 1,271.3 ± 245.3 b 12.1 ± 6.6 a 17.3 ± 4.3 a
SOX2 ** 2.5 ± 1.4 a 7.1 ± 0.8 a 4,800.8 ± 387.7 b 5.8 ± 3.0 a 3.0 ± 1.1 a
POU5F1 0.7 ± 0.3 a 0.6 ± 0.1 a 4.0 ± 1.0 a 142.5 ± 0.2 b 3.9 ± 2.2 a 0.3 ± 0.1 a
  1. ADIPOQ: adiponectin; AT-MSC: adipose tissue-derived MSC; BM-MSC: bone marrow-derived MSC; CEPBA: CCAAT/enhancer-binding protein alpha; d10: day 10; d21: day 21; MSC: mesenchymal stromal cells; POU5F1: POU domain, class 5, transcription factor 1; PPARG: peroxisome proliferator-activated receptor gamma; SOX2: SRY (Sex Determining Region Y)-Box 2; WJ-MSC: Wharton’s Jelly-derived MSC; *not detected in initial sample (d0); **not detected in that particular sample (d10 or d21).
  2. Negative values mean downregulation on mRNA expression when compared to non-induced cultured cells, and positive values, upregulation. Statistical significance was assessed by one-way non-parametric ANOVA followed by Bonferroni post-hoc test to compare all pairs of data for a determined gene (α = 0.05, n = 3). In a same row, a same letter means that both conditions are not significantly different; different letters (superscript letters a and b) indicate statistically different values.