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Table 1 Relative gene expression (expressed in mean ± standard deviation) for human adipose tissue- (AT-), bone marrow- (BM-) and Wharton’s Jelly-mesenchymal stromal cells (WJ-MSC) when grown in low glucose media supplemented with 10% fetal bovine serum

From: Gene expression and protein secretion during human mesenchymal cell differentiation into adipogenic cells

 

BM-MSC

AT-MSC

WJ-MSC

d10

d21

d10

d21

d10

d21

PPARG

14.9 ± 2.5 a

32.3 ± 3.0 a

21.0 ± 6.4 a

58.3 ± 13.6 b

15.2 ± 8.9 a

29.1 ± 3.2 a

ADIPOQ

4,126.8 ± 3,370.2 a

3,994.2 ± 1,292.7 a

3,378.4 ± 2,243.0 a

423,626.8 ± 147,352.8 b

*

*

CEBPA

102.6 ± 14.6 a

89.7 ± 33.1 a

206.6 ± 53.9 a

1,271.3 ± 245.3 b

12.1 ± 6.6 a

17.3 ± 4.3 a

SOX2

**

2.5 ± 1.4 a

7.1 ± 0.8 a

4,800.8 ± 387.7 b

5.8 ± 3.0 a

3.0 ± 1.1 a

POU5F1

0.7 ± 0.3 a

0.6 ± 0.1 a

4.0 ± 1.0 a

142.5 ± 0.2 b

3.9 ± 2.2 a

0.3 ± 0.1 a

  1. ADIPOQ: adiponectin; AT-MSC: adipose tissue-derived MSC; BM-MSC: bone marrow-derived MSC; CEPBA: CCAAT/enhancer-binding protein alpha; d10: day 10; d21: day 21; MSC: mesenchymal stromal cells; POU5F1: POU domain, class 5, transcription factor 1; PPARG: peroxisome proliferator-activated receptor gamma; SOX2: SRY (Sex Determining Region Y)-Box 2; WJ-MSC: Wharton’s Jelly-derived MSC; *not detected in initial sample (d0); **not detected in that particular sample (d10 or d21).
  2. Negative values mean downregulation on mRNA expression when compared to non-induced cultured cells, and positive values, upregulation. Statistical significance was assessed by one-way non-parametric ANOVA followed by Bonferroni post-hoc test to compare all pairs of data for a determined gene (α = 0.05, n = 3). In a same row, a same letter means that both conditions are not significantly different; different letters (superscript letters a and b) indicate statistically different values.