Figure 1
Biochemical characteristics of RTA DHF and RTA IHF . (A) Sequence of the C-terminal hydrophobic region of ricin A-chain. Introduced mutations are marked in red. (B) Hydrophobicity plot for the C-terminal region (Val245-Val256) of wild-type ricin A-chain, wt, DHF ricin A-chain and IHF ricin A-chain. Presented graphs are fragments of the output from ProtScale [64] prepared for full-length ricin A-chains. A sliding window of 13 AAs was used. Wild-type RTA (unbroken line); RTADHF (dashed line); RTAIHF (dotted line) (C) Far UV CD spectra of wild-type RTA, wt; RTADHF and RTAIHF. The scans were corrected by the subtraction of blanks containing only the buffer. Averaged spectra from three measurements; s.d. ≤ 3%. Wild-type RTA (unbroken line); RTADHF (dashed line); RTAIHF (dotted line).