Figure 7
BACE457 DHF is a membrane protein. (A) Cells were subjected to permeabilisation (see Methods) with increasing concentrations of digitonin, concentrations as indicated. A representative membranes of cytosolic fraction and membrane fraction after Western blot with anti-calnexin (clx) or anti-calreticulin (cln) antibodies are shown. (B) Cells were transfected with cDNA encoding BACE457, BACE457DHF or BACE457Δ and subjected to permeabilisation with increasing concentrations of digitonin, concentrations as indicated. A representative membranes of cytosolic fraction and membrane fraction after Western blot with anti-BACE antibodies are shown. (C) Cells were transfected with cDNA encoding BACE457, BACE457DHF or BACE457Δ and subjected to permeabilisation with 60 μg/ml of digitonin. Then cells were fixed and stained as indicated in Methods. Bars, 10 μm.