Decreased hydrophobicity of BACE457 impairs its interactions with EDEM1 and EDEM2. Co-immunoprecipitation of BACE457 or BACE457DHF with anti-HA antibodies from cells overexpressing EDEM1-HA (A) or EDEM2-HA (B). Representative examples of the experiments are shown. Membranes were re-probed with anti-HA antibodies to confirm equal immunoprecipitation. Whole cell lysates (WCL) analysed with anti-BACE antibodies are shown in the bottom panel. (C), (D) Graphs of the average data presented in (A) and (B) with s.d. from three independent experiments are shown. The level of immunoprecipitated BACE457 from EDEM1-transfected cells or EDEM2- transfected cells is marked as 1, the level of immunoprecipitated BACE457 DHF is relative to this value.