Mps1 with a single leucine substituted in pNES1 relocate to the nucleus. (A) pNES1 motif is adjacent to the LXXLL motifs within TPR domain. (B) The subcellular distribution of wild type Mps1, Mps1 S821A, Mps1mutNES1, Mps1deltaA domain at G2/M boundary. (C) The centrosome localization of wild type Mps1 and Mps1mutNES. (D) The subcellular distribution of wild type Mps1, Mps1mutNES1-1, Mps1mutNES1-2, Mps1mutNES1-3, Mps1mutNES1-4 in interphase.