Skip to main content
Figure 4 | BMC Cell Biology

Figure 4

From: A novel fluorescence-based biosynthetic trafficking method provides pharmacologic evidence that PI4-kinase IIIα is important for protein trafficking from the endoplasmic reticulum to the plasma membrane

Figure 4

Treatment with PAO inhibits the biosynthetic trafficking of EGFR to the PM in a dose-dependent manner . Live cells transiently expressing EGFR-GFP were processed as described for Figure 1, except that PAO was added at indicated concentrations to half of the cells at hour 3. (A) Flow cytometry was used to calculate the ratio of PM localized EGFR fluorescence to total EGFR fluorescence, and this ratio for each time point ± PAO (2.5 μM) is plotted. Error bars indicate ± SE of five independent experiments in which approximately 8,000 EGFR-GFP expressing cells were analyzed at each time point. *** p < 0.001 (B) Dose-dependence of PAO inhibition of biosynthetic trafficking of EGFR. Samples were processed as in A following addition of indicated concentrations of PAO. Data from one experiment is representative of 2 separate experiments; approximately 8,000 EGFR-GFP expressing cells were analyzed at each time point. (C) Confocal images of RBL cells expressing EGFR-GFP either untreated or treated with PAO, cells are from 8 hr time points quantified in A. Scale bar shows 5 μm.

Back to article page

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us