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Figure 5 | BMC Cell Biology

Figure 5

From: A novel fluorescence-based biosynthetic trafficking method provides pharmacologic evidence that PI4-kinase IIIα is important for protein trafficking from the endoplasmic reticulum to the plasma membrane

Figure 5

Analysis of biosynthetic trafficking by susceptibility to digestion by endoglycosidase H. RBL mast cells transiently expressing pkH3-EGFR were subjected to the same trafficking protocol as described in Figure 1. However, rather than processing samples for flow cytometry, whole cell lysates were collected and were either untreated (Un), treated with EndoH (EH), or treated with PNGase F (PNG) for 24 hr before Western blot analysis with an anti-EGFR antibody. The EGFR protein from the PAO treated cells is completely Endo H sensitive by 7 hr, thus supporting the contention that these EGFR proteins contain only N-linked oligosaccharide chains and have not trafficked beyond the ER.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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