Figure 3

Mitochondrial status in Calu-1 and A549 cells expressing 3Cpro. Cells maintaining mitochondrial transmembrane potential were visualized using the potential-dependent fluorescent dye rhodamine 123. A549/Mock and Calu-1/Mock cells 48 h p.t. (A). A549/3Cpro and Calu-1/3Cpro cells: vacuolated, 48 h p.t. (B, C) and 72 h p.t. (D, E); round, 72 h p.t. (F); with blebbed plasma membrane, 72 h p.t. (G). EGFP and Rhod are channels for GFP and rhodamine 123 fluorescence, respectively; eGFP + Rhod channel shows overlapping signals.