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Figure 1 | BMC Cell Biology

Figure 1

From: A novel myogenic function residing in the 5′ non-coding region of Insulin receptor substrate-1 (Irs-1) transcript

Figure 1

Characterization of two distinct Irs-1 transcripts. (A) Schematic representation of two transcriptional variants of mouse Irs-1 mRNAs (FL-Irs-1 and s5′-Irs-1 mRNAs). The positions of specific qPCR primer sets are shown as arrows (red is for FL-Irs-1, blue is for s5′-Irs-1 mRNA). (B) Expression levels of s5′-Irs-1 mRNA in the indicated mouse tissues, proliferating C2C12 myoblasts (GM, growth medium) and differentiating C2C12 myotubes (DM4, differentiation medium for 4 days). Mean ± SD, n = 4-6. (C) Expression levels of FL-Irs-1 mRNA in the indicated mouse tissues, C2C12 myoblasts (GM) and C2C12 myotubes (DM4). Mean ± SD, n = 4-6. (D,E) Specific knockdown of FL-Irs-1 mRNA (d) or s5′-Irs-1 mRNA (e) in myoblasts (GM) and myotubes (DM4). Mean ± SD, n = 4, *p < 0.01 versus no treatment (none). (F) Effect of specific knockdown of FL-Irs-1 mRNA or s5′ Irs-1 mRNA on IRS-1 protein expression during myogenesis. (G) Quantification analysis and statistic were performed on three independent experiments. Mean ± SD, *p < 0.01 versus each culture conditions (GM, DM1 or DM3) in no transfection (none). n.s., not significant.

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