Figure 4

FL- Irs-1 transcript does not reduce Rb mRNA expression in hepatocytes. (A) Effect of FL-Irs-1 5′UTR on Rb mRNA expression levels. Cultured hepatocyte cell lines (NCTC clone 1469) were untransfected (−) or transfected with an Rb mRNA-expressing plasmid in combination with the indicated effector plasmid. Rb mRNA levels were measured by qRT-PCR. Mean ± SD, n = 4, *p < 0.01 versus control transfectant (pRB + pLuc). (B,C) Effect of knockdown of endogenous FL-Irs-1 transcript on endogenous Rb mRNA levels. Cultured hepatocyte cell lines were transfected with the indicated shRNA. FL-Irs-1 (B) and Rb mRNA (C) levels were measured by qRT-PCR. Mean ± SD, n = 4, *p < 0.01 versus no treatment (none).