Figure 5

Dex addition in standard osteogenic medium for the last 2 days has no effect on osteoblast differentiation. Relative levels of mRNA osteoblast markers (Runx2, OSX (osterix), ALP (alkaline phosphatase), OC (osteocalcin)) were determined on BMSCs cultured for 14 days in growth medium (G 0), in adipogenic medium containing Dex 500 nM (A 500), or cultured in osteogenic medium (O 0) or osteogenic medium supplemented with 100 nM of Dex (O 100) for 14 days or for the last 2 days (O 100(2d)). Results were reported to expression of the mean of Gapdh2 and 18S and expressed in percent of cells cultured in growth medium. Representative data from four independent experiments depicted as means +/− S.E.M. are shown. ^# p < 0.05 vs. O 0 medium.