Figure 6

Dex addition in standard osteogenic medium did not affect cell proliferation. (A) Dex effect on cell growth was determined by measuring the mRNA expression of 3 cyclins (D1, E1 and B1) on BMSCs cultured for up to 14 days in growth medium (G 0), in adipogenic medium containing Dex 500 nM (A 500), or cultured in osteogenic medium (O 0) or osteogenic medium supplemented with 100 nM of Dex (O 100). Results were reported to expression of the mean of Gapdh2 and 18S and expressed in percent of cells cultured in growth medium. (B) Dex effect on cell growth was assessed by performing western blot analysis of cyclin D1 in the same conditions as in (A).