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Figure 7 | BMC Cell Biology

Figure 7

From: Dexamethasone in osteogenic medium strongly induces adipocyte differentiation of mouse bone marrow stromal cells and increases osteoblast differentiation

Figure 7

Characterization of mineralization nodules and lipid accumulation by Raman microspectroscopy in the co-differentiation medium. BMSCs cultured for 14 days in osteogenic, adipogenic (with Dex 500 nM) or co-differentiation media (100 nM of Dex added to osteogenic medium) were analyzed by Raman microspectroscopy. Optical images representative of three independent experiments performed in triplicate are shown. (A) Lipid accumulation of adipocytes in the adipogenic medium. (B) Mineralized nodules of apatitic calcium phosphate named here bioapatite (Bio-Ap) of cells cultured in the osteogenic medium. (C) Undifferentiated cells in the growth medium. (D) Lipid accumulation of adipocytes of cells cultured in the co-differentiation medium. (E) Mineralized nodules of Bio-Ap of cells cultured in the co-differentiation medium. (F) Reference Raman spectra of lipid accumulation of adipocytes in the adipogenic medium; (G) Reference Raman spectra of mineralized nodules of Bio-Ap in the osteogenic medium; (H) Reference Raman spectra of undifferentiated cell in the growth medium; (I) Raman spectra of lipid accumulation of adipocytes in the co-differentiation medium; (J) Raman spectra of mineralized nodules of Bio-Ap of cells cultured in the co-differentiation medium; (K) Reference Raman spectra of cell support. The position of the Raman spectrum is indicated by white arrow heads; a.u. = arbitrary units.

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