Figure 3
Morphologic observation and adipocyte-specific mRNA quantification of EVs isolated from CM obtained from adipocytes after 14 days of differentiation. (A) Images obtained by transmission electron microscopy on the EVs collected at day 14 of adipocyte differentiation of hMSC, after negative coloration by uranyl oxalate. Scale bars: 200 and 100 nm. (B) RT-qPCR analysis of PPARγ, Leptin, CEBPα and CEBPδ in EVs isolated from 0, 7, 14, and 21 days-differentiated adipocytes. Transcript levels of GAPDH were used for sample normalization.