Figure 4

Species-specific adipogenic mRNAs observation in osteoblasts incubated in different species adipocytes conditioned medium. (A) Agarose gel electrophoresis of RT-PCR products of hPPARγ, hLeptin, hCEBPδ, hGAPDH and h36B4 from 14 days-differentiated MC-3 T3 (MC3T3 D14) incubated with conditioned medium from 14 days-differentiated human adipocytes (hMSC-Adi D14 CM). Two representative experiments are shown (1 and 2). Positive control was performed on hMSC-Adi D14 and negative control was obtained by incubating MC3T3 D14 with serum- and inductors-free DMEM for the same time (ctrl). (B) Images obtained by optical microscopy on mouse bone marrow-derived adipocytes (mBM-Adi) after 14 days of differentiation. Scale bar: 100 μm. (C) Agarose gel electrophoresis of RT-PCR products of mPPARγ, mAdiponectin, mCEBPα, mGAPDH and mActin from 14 days-differentiated human osteoblasts (hMSC-Ost D14) incubated with conditioned medium from 14 days-differentiated mouse adipocytes (mBM-Adi D14 CM). Two representative experiments are shown (1 and 2). Positive control was performed on mBM-Adi D14 and negative control was obtained by incubating hMSC-Ost D14 with serum- and inductors-free DMEM for the same time (ctrl).