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Table 1 mRNA primer sequences

From: Adipogenic RNAs are transferred in osteoblasts via bone marrow adipocytes-derived extracellular vesicles (EVs)

mRNA

Primers

Accession numbers

Human specific primers

  

hPPARγ

F: 5′-GCTTCTGGATTTCACTATGG-3′

NM_005037

 

R: 5′-AAACCTGATGGCATTATGAG-3′

 

hLeptin

F: 5′-ATTTCACACACGCAGTCAGT-3′

NM_00230

 

R: 5′-GAAGAAGATCCCGGAGGT-3′

 

hCEBPα

F: 5′-ACTGGGACCCTCAGCCTTG-3′

NM_004364

 

R: 5′-TGGACTGATCGTGCTTCGTG-3′

 

hCEBPδ

F: 5′-ACGACGACGAGAGCGCCATC-3′

NM_005195

 

R: 5′-CGCCCGCCTTGTGATTGC-3′

 

hOC

F: 5′-ATGAGAGCCCTCACACTCCTC-3′

NM_199173

 

R: 5′-GCCGTAGAAGCGCCGATAGGC-3′

 

hOP

F: 5′-CCTGCCAGCAACCGAAGTTT-3′

NM_001040058.1

 

R: 5′-ACTGTCCTTCCCACGGCTGT-3′

 

hYWHAZ

F: 5′-GGTCATCTTGGAGGGTCGTC-3′

NM_145690

 

R: 5′-GTCATCACCAGCGGCAAC-3′

 

hGAPDH

F: 5′-GTTCCAATATGATTCCACCC-3′

NM_002046.5

 

R: 5′-AGGGATGATGTTCTGGAGAG-3′

 

h36B4

F: 5′-CGACCTGGAAGTCCAACTAC-3′

NM_001002.3

 

R: 5′-AGCAACATGTCCCTGATCTC-3′

 

Mouse specific primers

  

mPPARγ

F: 5′-TTTTCAAGGGTGCCAGTTTC-3′

NM_001127330

 

R: 5′-AATCCTTGGCCCTCTGAGAT-3′

 

mAdiponectin

F: 5′-CCCAGTCATGCCGAAGA-3′

NM_009605.4

 

R: 5′-TACATTGGGAACAGTGACGC-3′

 

mCEBPα

F: 5′-TTACAACAGGCCAGGTTTCC-3′

NM_007678

 

R: 5′-CTCTGGGATGGATCGATTGT-3′

 

mGAPDH

F: 5′-GGCATTGCTCTCAATGACAA-3′

NM_008084

 

R: 5′-TGTGAGGGAGATGCTCAGTG-3′

 

mActin

F: 5′-AATTTCTGAATGGCCCAGGT-3′

NM_007393

 

R: 5′-GGTAAGGTGTGCACTTTTATTGG-3′

 
  1. Shown are the primer sequences and Genbank accession numbers. F: forward; R: reverse. h: human; m: mouse. PPARγ, peroxisome proliferator-activated receptor gamma; CEBPα and δ, CCAAT/enhancer binding protein alpha and delta; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5- monooxygenase activation protein, GAPDH, Glyceraldehyde 3-phosphate dehydrogenase. Human and mouse primers for PPARγ amplified isoforms 1 and 2.