Figure 2

Gating strategy for flow cytometry analysis of dissociated cells. A & F. Density plot displaying Side scatter versus Forward scatter before (A) and after (F) compensation for autofluorescence. B & G. Representation of Hoechst staining in dot plots versus Side scatter before (B) or after (G) compensation for autofluorescence. C & H Representation of Hoechst staining in dot plots versus Hoechst Green signal before (C) or after (H) compensation for autofluorescence. The compensation breaks the correlation between SSC or Hoechst’s green signal with Hoechst’s blue signal. D & I. Representation of the cell cycle before (D) and after (I) compensating for autofluorescence. E & J. The first peak corresponds to G1 cycle cells, whereas the second peak to the G2 cycle cells, with cells in phase S in between. Compared to non- compensated sample (E), compensation (J) enhances the precision of the cell cycle study.