Figure 3
Immunofluorescence analysis of control (A, C, E) and fibrotic kidneys (B, D, F) at magnification x20. Prominin-1 (red) staining is shown in A and B, Megalin (green) staining is shown in C and D, and double staining is shown in E and F. Expression of megalin and prominin1 colocalize on the brush border of renal proximal tubular epithelium in mice. In fibrotic kidneys, tubules are interspaced with fibrosis but still express Prominin-1 and Megalin.