Figure 7

RT-PCR analysis of Prominin-1 ⁺ cultured cells. A) Expression of Collagen-1, Ctgf, Megalin, Cd31 and Prominin-1 mRNAs in a primary culture of Prominin-1⁺ cells. Prominin-1, Megalin and Ctgf are strongly expressed as expected in proximal tubular cells, whereas Collagen 1 and Cd31 are virtually absent. B) Effect of TGFß on Prominin-1, Megalin, Collagen-1 and Ctgf mRNAs expression. TGFß upregulated Ctgf and Collagen-1 mRNAs, downregulated Megalin mRNA, and Prominin-1 mRNA remained stable.