MyoD expression in miRNA transfectants from day 1 to day 3 after transfection and medium change. The nuclear staining pattern is shown (Green: Myo D, Blue: DAPI, photos were taken from the area indicated in the white rectangular). MyoD expression was observed in both the cytoplasm and nucleus. Scale bar 50 μm. MyoD nuclear staining was counted and is presented in the bar graph. There were no significant differences in MyoD nuclear positive ratios. NC: negative control.