The expression of myogenin in miRNA transfectants on day 3 after transfection. Expression of myogenin was observed in the cytoplasm and nucleus. The nuclear staining pattern is shown (Green: myogenin, Blue: DAPI, photos were taken from the area indicated in the white rectangular). Nuclear myogenin expression was counted. There were no significant differences in myogenin nuclear positive ratios. Scale bar 50 μm. NC: negative control.