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Table 1 Primers sequence used for qRT-PCR analysis

From: In vitro analysis of the effects on wound healing of high- and low-molecular weight chains of hyaluronan and their hybrid H-HA/L-HA complexes

Gene

Forward primer

Reverse primer

Thermal Cycles

Transforming growth factor, beta 1 (TGFβ-1)

5′TgCggCAgCTgTACATTgA ′3

5′TggTTgTACAgggCCAggA′3

95 °C 10 s, 55 °C 30 s, 72 °C 3 min, 40 cycles

Collagen, type I, alpha 1 (COL1A1)

5′CAgCCgCTTCACCTACAg C′3

5′TTTTgTATTCAATCACTgTCTTgCC′3

94 °C 1 min, 56 °C 2 min, 72 °C 3 min, 40 cycles

Matrix metallopeptidase 2 (MMP-2)

5′gCCgCCTTTAACTggAgCAA′3

5′TTCCAggCATCTgCgATgAg′3

95 °C 10 s, 60 °C 30 s, 72 °C 3 min, 40 cycles

Matrix metallopeptidase 9 (MMP-9)

5′gCgCCACCACAgCCAACTATg′3

5′TggATGCCgTCTATgTCgTCTTTA′3

94 °C 1 min, 56 °C for 2 min, 72 °C 3 min, 40 cycles

Matrix metallopeptidase 13 (MMP-13)

5′TCCCAggAATTggTgATAAAgTAgA′3

5′CTggCATgACgCgAACAATA′3

95 °C 10 s, 55 °C 30 s, 72 °C 3 min, 40 cycles