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Table 1 Primers sequence used for qRT-PCR analysis

From: In vitro analysis of the effects on wound healing of high- and low-molecular weight chains of hyaluronan and their hybrid H-HA/L-HA complexes

Gene Forward primer Reverse primer Thermal Cycles
Transforming growth factor, beta 1 (TGFβ-1) 5′TgCggCAgCTgTACATTgA ′3 5′TggTTgTACAgggCCAggA′3 95 °C 10 s, 55 °C 30 s, 72 °C 3 min, 40 cycles
Collagen, type I, alpha 1 (COL1A1) 5′CAgCCgCTTCACCTACAg C′3 5′TTTTgTATTCAATCACTgTCTTgCC′3 94 °C 1 min, 56 °C 2 min, 72 °C 3 min, 40 cycles
Matrix metallopeptidase 2 (MMP-2) 5′gCCgCCTTTAACTggAgCAA′3 5′TTCCAggCATCTgCgATgAg′3 95 °C 10 s, 60 °C 30 s, 72 °C 3 min, 40 cycles
Matrix metallopeptidase 9 (MMP-9) 5′gCgCCACCACAgCCAACTATg′3 5′TggATGCCgTCTATgTCgTCTTTA′3 94 °C 1 min, 56 °C for 2 min, 72 °C 3 min, 40 cycles
Matrix metallopeptidase 13 (MMP-13) 5′TCCCAggAATTggTgATAAAgTAgA′3 5′CTggCATgACgCgAACAATA′3 95 °C 10 s, 55 °C 30 s, 72 °C 3 min, 40 cycles