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Table 1 Primer pairs for qPCR on HL-1 cell and mouse brain cDNA

From: Distinct expression patterns of HCN channels in HL-1 cardiomyocytes

Protein

Gene

Primer

Sequence (5‘→3‘)

T m

Amplicon size

GAPDH

gapdh

Forward

GGTATCGTGGAAGGACTCATG

62 °C

150 bp/284 bp

Reverse

GCTGCCAAGGCTGTGGGC

HCN1

hcn1

Forward

ACTGTGGGCGAATCCCTGG

62 °C

184 bp

Reverse

CCACCAGCAGCTGTGCAGA

HCN2

hcn2

Forward

GGAGAATGCCATCATCCAGG

62 °C

149 bp

Reverse

CAGCAGGCTGTGGCCATGA

HCN3

hcn3

Forward

TGGGAACCACTGGTGCACG

62 °C

141 bp

Reverse

TGAGCGTCTAGCAGATCGAG

HCN4

hcn4

Forward

CACGACCTCAACTCAGGCG

62 °C

153 bp

Reverse

CAGCGGGGTCCATATAACAG

  1. Primer sequences and amplicon sizes are based on mouse sequences (accession numbers: NM_008084.2 (gapdh), NM_010408.3 (hcn1), NM_008226.2 (hcn2), NM_008227.1 (hcn3), and NM_001081192.1 (hcn4)). Amplicon sizes of gapdh fragments are indicated for cDNA (150 bp) and genomic DNA (284 bp).