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Fig. 3 | BMC Cell Biology

Fig. 3

From: Extracellular fluid flow and chloride content modulate H+ transport by osteoclasts

Fig. 3

Changes in intracellular pH (pHi) of osteoclasts. The pHi of primary osteoclasts labeled with the pH-sensitive dye BCECF-AM (12 μM) was monitored along time in order to evaluate the H+ secretion. a. Primary osteoclast exhibit rhythmic fluctuations in pHi after the NH4Cl prepulse under non-flowing standard HEPES-buffered solution. b. Different patterns of pHi oscillations are observed in the primary osteoclasts under non-flowing standard HEPES-buffered solution, with variations in period and amplitude in cycles of acidification and alkalinization, in the presence or absence of NH4Cl prepulse. Each line represents one osteoclast

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