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Fig. 4 | BMC Cell Biology

Fig. 4

From: Three-dimensional poly-(ε-caprolactone) nanofibrous scaffolds directly promote the cardiomyocyte differentiation of murine-induced pluripotent stem cells through Wnt/β-catenin signaling

Fig. 4

PCL nanofibrous scaffolds promote the cardiomyocyte differentiation of miPSCs. a Co-labeling of the spontaneously differentiated miPSC cardiomyocyte (CM) monolayer after 15 days with cTnT and MLC2a antibodies. Scale bar, 20 μm. b Flow cytometric analysis of cells from TCP and (c) PCL scaffold monolayer culture for cTnT at 15 days differentiation. n = 3 independent experiments. dg Quantitative RT-PCR analysis of gene expression in the PCL and TCP groups. Total RNA was isolated from cells in TCPs and on 3D PCL nanofibrous scaffolds at 4 days after differentiation for MESP1 (d) analysis and at 10 days after differentiation for NKX2.5 (e), GATA4 (f) and TNNT2 (g) analyses. The expression levels of each gene were normalized to the endogenous control GAPDH. The fold changes relative to TCPs from triplicate experiments are presented. **Significantly different between the PCL and TCP groups, **P < 0.01. h Representative immunoblots of cTnT, α-actinin and GAPDH in mouse neonatal CMs, miPSC-derived cells on PCL scaffolds and in TCPs. i Quantification of cTnT and (j) α-actinin protein levels in mouse neonatal CMs, miPSC-derived cells on PCL scaffolds and in TCPs. **Significantly different between the PCL or TCP group versus the CM group, ##Significantly different between the PCL and TCP groups, **## P < 0.01. n = 5. All values are presented as the mean ± SE

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