Fig. 2

Overexpression of miR-34a inhibited proliferation, migration and invasion of JEG-3 cells. MiR-34a and non-targeting control (NC) sequence were transfected into JEG-3 cells. a The level of mature miR-34a was determined by real-time PCR analysis 24 h after transfection. b At 24 h after transfection, the cells were subjected to MTT assay to assess cell proliferation with 5 replicates for each time point. c Cell migration was measured by scratch wound assay. The cells were photographed at 100× magnification at 0 h and 24 h post-scratching, and the scale bars indicate 100 μm. d At 24 h after transfection, the cells were set up for in vitro Matrigel-based invasion assay to assess cell invasiveness. Following 24 h incubation, the invading cells on the bottom surface of the Transwell membrane were fixed, stained and photographed at 200× magnification (100 μm scales), and the average number of invading cells of 5 randomly selected fields from each sample were calculated. Each experiment was performed at least three times. The figure shows the representative images from repeated experiments and the values are expressed as the mean ± standard deviation. Compared with parental JEG-3 cells, *p < 0.05; **p < 0.01; ***p < 0.001