Skip to main content
Fig. 3 | BMC Cell Biology

Fig. 3

From: MicroRNA-34a inhibits human trophoblast cell invasion by targeting MYC

Fig. 3

MYC regulates the invasion of JEG-3 cells. JEG-3 cells were transfected with MYC overexpression construct, MYC siRNA, or the corresponding empty vectors. 48 h later, the levels of a MYC mRNA and b MYC protein in the cells were analyzed by real-time PCR and Western blot analysis respectively, using β-actin as the internal control. c Matrigel-based Transwell invasion assay was set up 24 h after transfection, and the number of invading cells was counted 24 h later at 200× magnification (scale bars = 100 μm). This figure shows the representative images from three independent experiments, and the values are expressed as the mean ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001

Back to article page