Fig. 4
From: Extended 2D myotube culture recapitulates postnatal fibre type plasticity
Extended myotube cultures exhibited phenotype plasticity and postnatal fibre phenotype. a Full complement of pre- and post-natal MyHC RNA isoforms in myotubes at 7, 15, 21 and 28 days of differentiation were quantified by real-time PCR (normalised to 18S rRNA). Use of DM1 (with Ultroser G) and DM2 (with horse serum) resulted in divergent MyHC profiles from day 15 of differentiation. MyHC profiles shown are a representative set of three independent experiments. b The use of DM1, but not DM2, promoted myotube transition towards a fast-glycolytic MyHC profile that resembled postnatal longissimus dorsi (LD) muscle of a 22-week-old Duroc pig. Error bars = standard deviation; ** = P ≤0.01, *** = P ≤0.001 based on two-sample unpaired t test at indicated time points