Fig. 5
From: Extended 2D myotube culture recapitulates postnatal fibre type plasticity
Induction of linc-MYH expression paralleled elevated expression of fast MyHC 2x and 2b in myotubes. The expression of linc-MYH (a), Six1 (b) and Eya1 (c) was determined by real-time PCR (normalised to 18S rRNA) in extended myotube cultures in DM1 (with Ultroser G) and DM2 (with horse serum), and skeletal muscles: fast longissimus dorsi (LD) and slow psoas (Ps). Skeletal muscle data shown are the combined results of three sets of LD and Ps from three 22-week-old pigs. Error bars = standard deviation; * = P ≤0.05, *** = P ≤0.001, **** = P ≤0.0001 based on two-sample unpaired t test at indicated time point