Fig. 3
From: EGF-induced sodium influx regulates EGFR trafficking through HDAC6 and tubulin acetylation
Intracellular sodium levels regulate vesicle trafficking. a DAOY cells were preincubated with either High Na+ or Low Na+ buffer for 30 minutes and then stimulated with 40 ng/ml of AlexaFluor 488-conjugated EGF for an additional 15 minutes during which time epifluorescence images were taken with a cooled CCD camera at a rate of one frame/second for a total of 900 frames. The motility of EGF-positive vesicles was analyzed using Slidebook software and average speed, maximum speed and end/total displacement were plotted. *, P < 0.0001. b EGF was added to DAOY cells incubated in buffers of increasing sodium concentrations and the motility of EGF-positive vesicles was analyzed as in A.