Fig. 4
From: EGF-induced sodium influx regulates EGFR trafficking through HDAC6 and tubulin acetylation
Increased acetylated tubulin decreases the motility of EGF-positive vesicles. a DAOY cells were transiently transfected with either wild-type tubulin (WT) or a nonacetylated mutant of tubulin (K40A). AlexaFluor 488-EGF was added in High Na+ buffer and images were acquired as described above. Average speed, maximum speed and the end/total displacement are shown. *, P < 0.001. b DAOY cells were preincubated with 100 nM TSA for 15 minutes and then AlexaFluor 488-EGF was added. Vesicles were tracked as described in figure legend 3. *, P < 0.001.