Fig. 6
From: EGF-induced sodium influx regulates EGFR trafficking through HDAC6 and tubulin acetylation
Acetylated tubulin mediated by intracellular sodium level regulates vesicle trafficking between endosomes. a DAOY cells were treated with AlexaFluor 488-EGF for 30 minutes a, b) or 60 minutes (C, D) in High Na+ or Low Na+ buffer. Immunofluorescence of early endosomes (EEA1) (A) and late endosomes (LAMP2) c are shown. The squares depict the areas of higher magnification. Scale bars represent 10 μm (low magnification) and 1 μm (high magnification). (B and D) The colocalization ratio was calculated using ImageJ and plotted as mean with standard errors.