Skip to main content
Fig. 3 | BMC Cell Biology

Fig. 3

From: The expression of B23 and EGR1 proteins is functionally linked in tumor cells under stress conditions

Fig. 3

Identification of the EGR1-binding sequence required for B23 promoter induction. a The human B23 promoter sequence, partial. An EGR1 binding motif is underlined at position −172 from the TATA box. A schematic representation of the WT sequence of B23 promoter and a copy with a mutated EGR1 binding site cloned upstream of Renilla luciferase gene (pB23-Luc, pB23-mut-Luc). b The luciferase assay in HeLa cells transfected with EGR1-GFP or the control GFP in combination with either of the two luciferase vectors (pB23-Luc or pB23-mut-Luc) plus a beta-galactosidase vector for normalization of transfection efficiency. All experiments were done under serum deprivation conditions. c Real time PCR of HeLa cells extracts either transfected with full length EGR1 or control GFP were incubated with DNA fragments amplified from the promoter sequence of B23 gene, in presence or absence of anti-EGR1 antibody

Back to article page