Fig. 5

EGR1 protein level is linked to B23. Analysis of EGR1 after B23 siRNA in HeLa cells grown at 0.2 % FBS. a Immunoblotting of EGR1 and B23 in whole extracts of HeLa cells after B23 siRNA. EGR1 protein significantly decreases following endogenous B23 silencing at 30 nM. b-c-d-e Real time PCR analysis of B23, EGR1, p300 mRNA and 47S pre-rRNA under B23 silencing. The level of expression of EGR1 remained unchanged (c). As expected p300 mRNA decrease (d) while the 47S rRNA levels increase levels (e). f-g Immunoblotting of EGR1 under MG-132 treatment after B23 siRNA for 16, 20 and 24 h. The level of EGR1 protein is restored during B23 silencing after treatment with proteosome inhibitor at 10 μM for 16 h at 37 ° C. Comparison tests were performed by one way ANOVA and significant results are highlighted with asterisks (*p < 0.05; **p < 0.01; ***p < 0.001)