Fig. 3

Internal deletions within the γA3 VCD motif affect association with full length γA3. a Six amino acid deletions in the γA3 VCD motif within the context of the VCD stub. b Coprecipitation of the VCD stub deletions with full length γB2. Deletions ΔD-F, ΔE-A, and ΔL-P significantly reduced association while other deletions had little or no effect. c Colocalization of VCD stub deletions with γB2. The γA3 VCD stub colocalized with full length γB2 in cells (arrowheads). Mutations that reduced VCD stub coprecipitation exhibited less colocalization with full length γB2 and could be found diffusely at the cell surface (double arrows)