Skip to main content
Fig. 5 | BMC Cell Biology

Fig. 5

From: The clustered protocadherin endolysosomal trafficking motif mediates cytoplasmic association

Fig. 5

a VCD motif deletions affect full length γA3 trafficking. Mutations that reduced (ΔE-A) or did not affect (ΔG-H) VCD association were generated within the context of full length γA3-GFP and evaluated for intracellular trafficking by correlative light and electron microscopy. Another mutation outside the VCD motif (ΔI-C) was also tested. b ΔE-A caused the molecule to accumulate in wavy structures with no multivesicular bodies observed. ΔG-H was associated with wavy membranes (arrow) as well as distorted enlarged multivesicular bodies (arrowhead). In contrast, a deletion outside the VCD motif (ΔI-C) allowed correct trafficking to multivesicular bodies (arrowhead) and tubules (arrow) previously observed for intact γA3. Bar =200 nm

Back to article page