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Fig. 4 | BMC Cell Biology

Fig. 4

From: Dissecting seipin function: the localized accumulation of phosphatidic acid at ER/LD junctions in the absence of seipin is suppressed by Sei1pΔNterm only in combination with Ldb16p

Fig. 4

Manipulation of single metabolic enzymes do not eliminate PA puncta. a Diagram of pathways leading to PA production and use. b, c Genetic manipulations were performed in wt and sei1Δ strains, each expressing mCherry behind OPI1 ORF in the chromosome. b The indicated (at left) enzymes producing PA were knocked out. All resulting strains still produced PA puncta. c The two enzymes that metabolize PA, Pah1p and Cds1p, were overexpressed on plasmids using the PGK1 promoter. Neither resulting strain attenuated the PA puncta. Scale bars, 5 μm

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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