Fig 5
Sei1ΔNterm suppresses puncta but only in the presence of overexpressed Ldb16p. a BODIPY staining of droplets in the indicated yeast strains. The multiple and less distinct droplets in the ΔΔ + Sei1p + Ldb16p strain is shown in greater magnification at the bottom. b Quantification of cells with supersized (>1 μm diameter) droplets in the indicated strains. Three fields of >240 cells/field were counted from two independent experiments. Error bars signify SEM. Lines above the bars indicate statistical difference between the indicated pairs, based on the Turkey range test in conjunction with ANOVA. *, p < 0.05; **, p <0.01. c Human seipin (BSCL2) suppresses PA-puncta in the ΔΔ strain. d PA-puncta in sei1Δ Opi1-GFP (BY4742) are not suppressed by Sei1ΔNterm-mCherry. An overexpressed mCherry fusion was used to illustrate the correct ER targeting. e Puncta are absent with the combination of Sei1ΔNterm and Ldb16. Green, BODIPY493/503; red, Opi1-mCherry. f Overexpressed Ldb16 in the ΔΔ background increases TAG but not SE levels. TAG and SE in the W303 wild type control strain set at 100 %. Bars and error bars represent mean + range from two independent experiments. Lines above the bars indicate statistical difference between the indicated pairs, based on the Turkey range test in conjunction with ANOVA. **, p <0.01; ***, p < 0.001. Scale bars, 5 μm