Table 2 A list of plasmids used in this study

pRS426 GFP-Spo20(51–91)

pRS426 expressing GFP fused to Spo20(51–91) which is sensing PA

Nakanishi et al., 2004 [23]

YCpLac111-Pah1-GFP

PAH1-GFP under control of the PAH1 promoter into CEN/LEU2 vector

Karanasios et al., 2010 [25]

YCpLac111-Pah1-D398AD400A-GFP

PAH1[D398A D400A]-GFP under control of the PAH1 promoter into CEN/LEU2 vector

Karanasios et al., 2010 [2])

pRS315 PGK1

Yeast centromeric expressing plasmid (YCp) with LEU2 marker carrying PGK1 promoter/ terminator

Binns et al., 2006 [36]

pRS315 PGK1-CFP-HDEL

pRS315 expressing ER luminal CFP marker

Szymanski et al., 2007 [12]

pRS315 PGK1-Pah1

pRS315 expressing Pah1 under control of PGK1 promoter/terminator

Adeyo et al., 2011 [37], This study

pRS 315 PGK1-Cds1

pRS315 expressing Cds1 under control of PGK1 promoter/terminator

This study

pRS 315 PGK1-Dgk1

pRS 315 expressing Dgk1 under control of PGK1 promoter/terminator

This study

pRS315 PGK1-mCherry

pRS315 expressing mCherry under control of PGK1 promoter/terminator

Cartwright et al., 2015 [14]

pRS315 PGK1-Sei1-mCherry

pRS315 expressing Sei1-mCherry under control of PGK1 promoter/terminator

Cartwright et al., 2015 [14]

pRS-315 Sei1^ΔNterm-mCherry

pRS315 expressing Sei1^ΔNterm-mCherry under control of PGK1 promoter/terminator

Cartwright et al., 2015 [14]

pRS315 PGK1-Ldb16

pRS315 expressing Ldb16 under control of PGK1 promoter/terminator

This study

pRS315 PGK1-Sei1

pRS315 expressing Sei1 under control of PGK1 promoter/terminator

Szymanski et al., 2007 [12]

pRS315-BSCL2

pRS315 expressing BSCL2 (short isoform) under control of PGK1 promoter/terminator

Szymanski et al., 2007 [12]

pRS316 PGK1-Sei1-TAP

pRS316 expressing SEI1-TAP fusion protein under control of PGK1 promter/terminator. TAP sequence was amplified from HBH TAP tag (HBH-KanMX6), a gift from Anne Spang (Biozentrum,Basel)

Tagwerker et al., 2006, This study

pRS316 PGK1-Sei1^ΔNterm -TAP

pRS316 expressing SEI1^ΔNterm -TAP fusion protein under control of PGK1 promter/terminator.

This study

pRS316 PGK1-Ldb16-Sei1-TAP

pRS316 expressing Ldb16-Sei1-TAP fusion protein under control of PGK1 promter/terminator. TAP seq was generated similar way to the above.

This study

pRS316 PGK1-Ldb16-Sei1^ΔNterm -TAP

pRS316 expressing Ldb16-Sei1^ΔNterm -TAP fusion protein under control of PGK1 promter/terminator. TAP seq was generated similar way to the above.

This study