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Fig. 2 | BMC Cell Biology

Fig. 2

From: Characterization of HAF-4- and HAF-9-localizing organelles as distinct organelles in Caenorhabditis elegans intestinal cells

Fig. 2

HAF-4- and HAF-9-localizing intestinal organelles are irrelevant to the storage and distribution of yolk granules. a Localization of HAF-4::GFP (green) and HAF-9::mCherry (magenta) to the intestinal granules in the day-1 adult hermaphrodite (top) and male (bottom) worms of Is[haf-4::GFP];Is[haf-9::mCherry]. The merged images and the corresponding DIC images are also presented. HAF-4::GFP and HAF-9::mCherry colocalized on the surface of intestinal granules not only in the hermaphrodites but also in the males. Bar, 5 μm. b Comparison of the localization of HAF-9::mCherry with VIT-2::GFP in Is[vit-2::GFP];Is[haf-9::mCherry]. VIT-2::GFP (green), HAF-9::mCherry (magenta), the merged image, and the corresponding DIC image are presented. VIT-2::GFP did not localize to the HAF-9::mCherry-positive intestinal granules. Dashed lines indicate the boundary of the intestine. Bar, 5 μm. c The localization of VIT-2::GFP in the wild-type (left) and haf-4(ok1042)haf-9(gk23) (right) worms. VIT-2::GFP and the merged images with corresponding DIC images are presented. Developing embryos positive for VIT-2::GFP are indicated by asterisks. Distribution of VIT-2::GFP to the embryos was unaltered even in the mutant worms. Bar, 10 μm

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