Fig. 5
From: Adopting the rapamycin trapping assay to track the trafficking of murine MHC class I alleles, H-2Kb
Cycling of Venus-FRB-Kb from post-ER compartment back to the ER. Venus-FRB-Kb is trapped in the ER by Cerulean-FKBP-Ii upon addition of rapaymcin. The fluorescence intensity of Venus-FRB-Kb accumulation from the first panel disappears upon the addition of rapamycin (1 mM) for 2 h (second panel). Trapping of Venus-FRB-Kb molecules does not occur in the controls where rapamycin is absent (first and third panels) or in the presence of Cerulean-Iip35 that lacks the FKBP domain (fourth panel). Scale bars, 10 μm