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Fig. 8 | BMC Cell Biology

Fig. 8

From: Adopting the rapamycin trapping assay to track the trafficking of murine MHC class I alleles, H-2Kb

Fig. 8

Preliminary data on the trafficking of H-2Kb from post-ER compartment directly to the cell surface. MEF cells were double transfected with Venus-FRB-Kb and Cerulean-FKBP-GPI. The invariant chain Ii is retained in the ER, whereas GPI can reach the cell surface. Cells treated with cycloheximide and rapamycin without addition of peptides showed Venus-FRB-Kb in the ER with a faint delineation of the cell surface. From the intensity RGB profile, the pseudo-color curves do not overlay. Cells treated with 10 μM of SIINFEKL peptides showed a clear cell surface stain that was more intense in the presence of rapamycin. The RGB profile depicts an overlay of the fluorophore curves at the same distance. Scale bars, 10 μm

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