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Fig. 2 | BMC Cell Biology

Fig. 2

From: Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells

Fig. 2

The Ra domain of TalinB interacts with Rap1 in a nucleotide dependent manner. a Pull down experiment with active and inactive GST-Rap1 protein as bait and purified RA domain of TalinB (RA-TalB) as prey. RA-TalB is precipitated with activated Rap1 but not with inactive Rap1 or GST control. b The dissociation rate of mGppNHp from Rap1 was measured in the presence of varying concentration of RA-TalB. These data were used to calculate the observed rate constants, kobs. The kobs values were plotted against the indicated effector concentration of RA-TalB. The addition of increasing concentrations of the effector results in a concentration dependent decrease of kobs. These data were used to calculate the dissociation constant of the Rap1GTP/ RA-TalB

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