Fig. 2

Types of TTs formed between HeLa cells. a and b A typical view of Cx43-EGFP- and Cx36-EGFP-expressing HeLa cells, respectively, exhibiting multiple fluorescent GJ plaques. c and d Only F-actin-containing F-TTs. e and f F-actin- and α-tubulin-containing Fα-TTs. (G and g) TTs formed GJs at the Cx-expressing cell border (see green dots and a white arrow indicating Cx43-EGFP cluster)