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Table 1 Summary of properties of open-ended and GJ-containing TTs

From: The role of neural connexins in HeLa cell mobility and intercellular communication through tunneling tubes

Cx isoform L (μm) Number of TTs per mm2/
per 100 cellsc 		gT (nS) siRNA/AF488
permeability (×10−14 cm3/s)
TT/(−)GJ
(HeLa wt) 		16.3 ± 0.4
(n = 214) 		51 ± 8/
9.5 ± 1.3 		6.0 ± 1.1a
(n = 17) 		65 ± 38
(n = 5)
TT/
Cx36-EGFP 		17.0 ± 0.4
(n = 330) 		79 ± 9*/
30 ± 7.1* 		0.8 ± 0.2
(n = 16) 		– b
(n = 5)
TT/
Cx40-CFP 		15.9 ± 0.5
(n = 126) 		46 ± 6/
9.7 ± 1.8 		16.5 ± 2.8
(n = 20) 		4.4 ± 2.0
(n = 6)
TT/
Cx43-EGFP 		17.0 ± 1.1
(n = 96) 		23 ± 4*/
4.7 ± 0.7* 		8.8 ± 3.4
(n = 7) 		32 ± 17
(n = 4)
TT/Cx45 14.3 ± 1.0
(n = 68) 		5.7 ± 1.6*/
1.4 ± 0.3* 		4.1 ± 0.5
(n = 41) 		– b
(n = 8)
TT/Cx47 15.1 ± 0.5
(n = 112) 		27 ± 3*/
5.0 ± 0.9* 		3.0 ± 0.6
(n = 15) 		4.3 ± 1.9
(n = 6)
  1. “–” nonpermeable; the number of experiments is indicated in parentheses; the diameter of TTs did not depend on the isoform of expressed Cxs and it was 0.9 μm on average (n = 210; varied from 0.4 to 2.4 μm)
  2. * p < 0.05 compared with HeLa wt cells
  3. athe majority of junctions were closed-ended (53 out of 70); b Cx36-EGFP and Cx45 GJs were impermeable to siRNA/AF488; however, they were permeable to AF488 (6.3 ± 1.9 × 10−14 cm3/s (n = 5) and 6.4 ± 4.9 × 10−14 cm3/s (n = 4), respectively); cCell densities measured 36 h after seeding were 81 ± 5; 75 ± 6; 78 ± 3; 73 ± 7; 70 ± 3; and 79 ± 5 cells per region for wt; Cx36-EGFP-; Cx40-CFP-; Cx43-EGFP-; Cx45-; and Cx47-expressing HeLa cells, respectively
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BMC Molecular and Cell Biology

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